Anti-UCHL5IP/HAUS7 Antibody Picoband™

Product Name

Anti-UCHL5IP/HAUS7 Antibody Picoband™

View all UIP1 Antibodies

SKU/Catalog Number

A12338-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-UCHL5IP/HAUS7 Antibody Picoband™ catalog # A12338-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-UCHL5IP/HAUS7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12338-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human UCHL5IP/HAUS7 recombinant protein (Position: D18-Q333). Human HAUS7 shares 69% amino acid (aa) sequence identity with mouse HAUS7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A12338-1 is reactive to HAUS7 in Human

Applications

A12338-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

40.778kDa

Background of UIP1

This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of UCHL5IP/HAUS7 using anti-UCHL5IP/HAUS7 antibody (A12338-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCHL5IP/HAUS7 antigen affinity purified polyclonal antibody (Catalog # A12338-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCHL5IP/HAUS7 at approximately 46 kDa. The expected band size for UCHL5IP/HAUS7 is at 41 kDa.

Click image to see more details

Figure 2. IF analysis of UCHL5IP/HAUS7 using anti-UCHL5IP/HAUS7 antibody (A12338-1).
UCHL5IP/HAUS7 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-UCHL5IP/HAUS7 Antibody (A12338-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Click image to see more details

Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-UCHL5IP/HAUS7 antibody (A12338-1).
Overlay histogram showing MCF-7 cells stained with A12338-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UCHL5IP/HAUS7 Antibody (A12338-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For HAUS7 (Source: Uniprot.org, NCBI)

Alternative Names

26S proteasome-associated UCH37-interacting protein 1; expressed-Xq28STS protein; HAUS augmin-like complex subunit 7; HAUS augmin-like complex, subunit 7; UCH37 interacting protein 1; UCHL5 interacting protein; UCHL5-interacting protein; UCHL5IPHSXQ28ORF; UIP126S proteasome-associated UCH interacting protein 1; X-linked protein STS1769 HAUS7 UCHL5IP, UIP1 HAUS augmin like complex subunit 7 HAUS augmin-like complex subunit 7|26S proteasome-associated UCH37-interacting protein 1|X-linked protein STS1769

For more info on HAUS7, check out the HAUS7 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HAUS7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the HAUS7 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis